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Learn more. DOI: Maritza Escalona. Jaime A. Teixeira da Silva. Danilo T. Pina Morgado. Show more authors. In the global flower industry, gerbera Gerbera jamesonii Bolus ex Hooker f. Commercially, micropropagation is more important than seed or vegetative propagation. Factors such as immersion frequency 6, 8 and 12 h , additional ventilation 1 min every 2 h and the length of duration of shoot culture 14, 21, 28 and 35 days were tested.
Immersion every 8 h with additional ventilation produced more shoots with better morphology than the control. However, when shoots were immersed every 6 h, shoots had higher fresh and dry weight, but displayed hyperhydricity.
No differences in shoots were found when immersion frequency was 8 or 12 h. Shoots receiving additional air supply every 8 h had lower fresh and dry weight with an Plantlets were subsequently acclimatized. This protocol allows for the mass production of gerbera shoots in a simple TIB, with effective rooting and acclimatization.